General Information of This Optogenetic System (OS)
OS ID
OS00031
OS Name
Photoactivatable GAL promoter to control transcription system 2 (PA-GAL 2)
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Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Regulatory protein GAL4
Detail Info
Recruited protein Regulatory protein Gal4
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1] , [ 2]
Controlled Signal Pathway DNA transcription
Effect Activation
Application Control DNA transcription
Note Control protein production with light in any yeast background strain
Light Information Activation Wavelength 461 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity >1 milliwatts
Expression Information Expression Method Transfection
Cell Lines Yeast; Zebrafish
References
1 Light-mediated control of DNA transcription in yeast
2 Optogenetic control of transcription in zebrafish