Details of the Optogenetic Tool | OPTICS

General Information of This Photoreceptor (PR)
PR ID	PR00351
PR Name	Residue1-908 of Phytochrome B (PhyB (1-908))
Source of Species	Arabidopsis
Class	Phytochromes
UniProt ID	P14713
GenBank	NP_179469
PDB ID	4OUR
Sequence	MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQ YTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCM IAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFV AREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVR AISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIG LHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQ YMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEF LMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAA FLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMA VAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWE TAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELGAVAREMVRLI ETATVPIFAVDAGGCINGWNAKIAELTGLSVEEAMGKSLVSDLIYKENEATVNKLLSRAL RGDEEKNVEVKLKTFSPELQGKAVFVVVNACSSKDYLNNIVGVCFVGQDVTSQKIVMDKF INIQGDYKAIVHSPNPLIPPIFAADENTCCLEWNMAMEKLTGWSRSEVIGKMIVGEVFGS CCMLKGPDALTKFMIVLHNAIGGQDTDKFPFPFFDRNGKFVQALLTANKRVSLEGKVIGA FCFLQIPS
Components	Photoreceptor	Residue1-908 of Phytochrome B (PhyB (1-908))
	Complementary Protein	Phytochrome interacting factor 6; PIF6 (PIF6)				Detail Info
	Cofactor	Phycocyanobilin (PCB)				Detail Info

Complementary Protein Detail of This PR
Complementary Protein	Phytochrome interacting factor 6; PIF6 (PIF6)
UniProt ID	Q8L5W7
Sequence	MMFLPTDYCCRLSDQEYMELVFENGQILAKGQRSNVSLHNQRTKSIMDLYEAEYNEDFMK SIIHGGGGAITNLGDTQVVPQSHVAAAHETNMLESNKHVDDSETLKASSSKRMMVDYHNR KKIKFIPPDEQSVVADRSFKLGFDTSSVGFTEDSEGSMYLSSSLDDESDDARPQVPARTR KALVKRKRNAEAYNSPERNQRNDINKKMRTLQNLLPNSHKDDNESMLDEAINYMTNLQLQ VQMMTMGNRFVTPSMMMPLGPNYSQMGLAMGVGMQMGEQQFLPAHVLGAGLPGINDSADM LRFLNHPGLMPMQNSAPFIPTENCSPQSVPPSCAAFPNQIPNPNSLSNLDGATLHKKSRK TNR

Cofactor Detail of This PR
Cofactor Name	Phycocyanobilin (PCB)
PubChem CID	5280816
Structure
Formula	C33H38N4O6
Molecular Weight	586.7

Optogenetic System (OS) Consisting of This PR

OS00596

OS Name: Light-induced dimerization system 1 (LID 1)

[1] , [2]

Fusion protein: C terminal of GTPase HRas

Recruited protein: Tyrosine-protein kinase ZAP-70

OS00600

OS Name: Llight-inducible organelle-targeting system (LIOTS)

[3]

Functional protein: Phytochrome B

Recruited protein: Phytochrome interacting factor 3

OS00598

OS Name: Optogenetic activate gene expression system (Opto-AGE)

[4]

Fusion protein: Tetracycline repressor protein class D

Recruited protein: Tyrosine-protein kinase ZAP-70

OS00599

OS Name: Optogenetic control nucleo/cytoplasmatic partitioning system (Opto-NCP)

[4]

Fusion protein: Nuclear export sequence

Functional protein: Phytochrome B

Recruited protein: Tyrosine-protein kinase ZAP-70

OS00593

OS Name: Optogenetic intracellular transport system 1 (Opto-IT 1)

[5]

Recruited protein: Kinesin-1 heavy chain

Fusion protein: Ras-related protein Rab-11A

OS00594

OS Name: Optogenetic intracellular transport system 3 (Opto-IT 3)

[5]

Recruited protein: Kinesin-like protein KIF1A

Fusion protein: Ras-related protein Rab-11A

OS00595

OS Name: Optogenetic intracellular transport system 5 (Opto-IT 5)

[5]

Recruited protein: Protein bicaudal D homolog 2

Fusion protein: Ras-related protein Rab-11A

OS00597

OS Name: Optogenetic regulate Galpha activity system 1 (Opto-Galpha 1)

[6]

Recruited protein: Guanine nucleotide-binding protein G(q) subunit alpha

Functional protein: Phytochrome B

References

1	Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling
2	Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics
3	A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast
4	Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish
5	A Phytochrome-Derived Photoswitch for Intracellular Transport
6	Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems