Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00593
OS Name
Optogenetic intracellular transport system 1 (Opto-IT 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue1-908 of Phytochrome B (PhyB (1-908))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
Components
Photoreceptor: Residue1-908 of Phytochrome B (PhyB (1-908))
Complementary Protein: Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Recruited protein Kinesin-1 heavy chain
Detail Info
Fusion protein Ras-related protein Rab-11A
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF6 heterodimerization [ 1]
Controlled Signal Pathway Target cargo transportation
Effect Sensor
Application Intracellular protein transport
Note Dependence on PCB as a cofactor could be considered a limiting factor for this system
Light Information Activation Wavelength 625 nm
Process Reversible
Deactivation Wavelength 740 nm
Activation Time 500 ms
Deactivation Time 3.8 s
Light Intensity 20 microwatts per square centimeter(625nm); 400 microwatts per square centimeter(740nm)
Expression Information Expression Method Transfection
Cell Lines COS-7 and U2OS cells
References
1 A Phytochrome-Derived Photoswitch for Intracellular Transport