Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00597
OS Name	Optogenetic regulate Galpha activity system 1 (Opto-Galpha 1)		[1]
Photoreceptor (PR) PR Info	PR Name Residue1-908 of Phytochrome B (PhyB (1-908)) Source of Species Arabidopsis Class Phytochromes UniProt ID P14713 GenBank NP_179469 PDB ID 4OUR Components Photoreceptor: Residue1-908 of Phytochrome B (PhyB (1-908)) Complementary Protein: Phytochrome interacting factor 6; PIF6 (PIF6) Cofactor: Phycocyanobilin (PCB)
Complementary Protein	Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor	Phycocyanobilin (PCB)
Controlled Protein	Recruited protein	Guanine nucleotide-binding protein G(q) subunit alpha	Detail Info
Controlled Protein	Functional protein	Phytochrome B	Detail Info

OS Application Information (1)
Mechanism of Action	PhyB-PIF6 heterodimerization		[ 1]
Controlled Signal Pathway	cAMP production
Effect	Activation
Application	Red light induced Ca2+ release and cAMP increase
Note	Galpha achieves activation by translocation Galpha from the cytoplasm to the plasma membrane
Light Information	Activation Wavelength	660 nm
	Process	Reversible
	Deactivation Wavelength	735 nm or dark
	Activation Time	Seconds
	Deactivation Time	Seconds
	Light Intensity	0.15 milliwatts per square centimeter(660nmnm); 1.5 milliwatts per square centimeter(735nm)
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	Hela cells

References

Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems