Details of the Optogenetic Tool

Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells

Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems

A photoactivatable Cre-loxP recombination system for optogenetic genome engineering

Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells

An AND-Gated Drug and Photoactivatable Cre-loxP System for Spatiotemporal Control in Cell-Based Therapeutics

General Information of This Photoreceptor (PR)
PR ID	PR00135
PR Name	Negative Magnet (nMag)
Source of Species	Neurospora crassa
Class	LOV domain
Sequence	HTLYAPGGYDIMGYLDQIGNRPNPQVELGPVDTSCALILCDLKKDTPIVYASEAFLYMTC RSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAIDRNAEVQVEVVNFKKNGQ RFVNFLTMIPVRDETGEYRYSMGFQCETE
Components	Photoreceptor	Negative Magnet (nMag)
	Complementary Protein	Positive Magnet (pMag)	Detail Info
	Cofactor	Flavin adenine dinucleotide (FAD)	Detail Info
3D Structure
3D Structure	Click to Save PDB File

Complementary Protein Detail of This PR
Complementary Protein	Positive Magnet (pMag)
Sequence	HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALILCDLKQKDTPIVYASEAFLYMT GYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAIDRNAEVQVEVVNFKKNG QRFVNFLTMIPVRDETGEYRYSMGFQCETE

Cofactor Detail of This PR
Cofactor Name	Flavin adenine dinucleotide (FAD)
PubChem CID	643975
Structure
Formula	C27H33N9O15P2
Molecular Weight	785.5

Optogenetic System (OS) Consisting of This PR

OS00281

OS Name: Enhanced Magnets system (EMags)

[1]

Functional protein: Negative Magnet

Recruited protein: Positive Magnet

OS00280

OS Name: Optogenetic regulate Galpha activity system 2 (Opto-Galpha 2)

[2]

Recruited protein: Guanine nucleotide-binding protein G(q) subunit alpha

Functional protein: Negative Magnet

OS00279

OS Name: Photoactivatable split-Cre recombinase optogenetic system (PA-Cre)

[3]

Fusion protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase

Recruited protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase

OS00699

OS Name: Photoactivatable targeted gene editing system (paCas9-Survivin)

[4]

Functional protein: Negative Magnet

Recruited protein: Positive Magnet

OS00278

OS Name: Tamoxifen-gated photoactivatable split-Cre recombinase optogenetic system (TamPA-Cre)

[5]

Fusion protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase

Recruited protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase

References