Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00281
|
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| OS Name |
Enhanced Magnets system
(EMags)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Positive Magnet (pMag) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Functional protein | Negative Magnet | ||||
| Recruited protein | Positive Magnet | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | nMag-pMag heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Sensor | |||||
| Application | Protein recruitment to different organelles | |||||
| Note | An effective tool to optogenetically manipulate physiological processes over whole cells or in small subcellular volumes | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 2.8 ± 0.3 s | |||||
| Deactivation Time | 14 ± 0.6 s | |||||
| Light Intensity | 3 microwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Hela cells and COS7 cells | |||||