Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00279
|
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| OS Name |
Photoactivatable split-Cre recombinase optogenetic system
(PA-Cre)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Positive Magnet (pMag) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase | ||||
| Recruited protein | CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | nMag-pMag heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Tamoxifen dependent gene activation | |||||
| Effect | Activation | |||||
| Application | Light inducible DNA recombination in living systems | |||||
| Note | Ambient light has no effect on DNA recombination with PA-Cre in vivo in living mice | |||||
| Light Information | Activation Wavelength | 470 ± 20 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 30s | |||||
| Deactivation Time | Seconds to Minutes | |||||
| Light Intensity | 5 watts per square meter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Mouse liver cells | |||||