Details of the Controlled Protein

General Information of This Controlled Protein (CP)
CP ID CP00273
CP Name Erythrobacter litoralis 222 amino acid protein (EL222)
CP Type Functional protein
Common Name Light-activated DNA-binding protein EL222 (EL222) (LOV-HTH DNA-binding protein)
Organism Erythrobacter litoralis (strain HTCC2594)
UniProt ID Q2NB98
UniProt Entry LVHTH_ERYLH
KEGG eli:ELI_04755
Function Has reversible, light-dependent DNA-binding activity. Upon illumination an internal FMN-protein adduct is formed which changes the protein conformation so that the previously sequestered DNA-binding domain is free to bind DNA. Binds to sequences within in its own promoter when illuminated but not when it has been incubated in the dark.
Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDHPFTMGQDRPIDGSGAPGADDTRVEVQP
PAQWVLDLIEASPIASVVSDPRLADNPLIAINQAFTDLTGYSEEECVGRNCRFLAGSGTE
PWLTDKIRQGVREHKPVLVEILNYKKDGTPFRNAVLVAPIYDDDDELLYFLGSQVEVDDD
QPNMGMARRERAAEMLKTLSPRQLEVTTLVASGLRNKEVAARLGLSEKTVKMHRGLVMEK
LNLKTSADLVRIAVEAGI
Optogenetic System (OS) Controlling This CP
OS00253
OS Info
OS Name
Enhenced blue light inducible promoter vision 1
[1], [2]
Photoreceptor (PR) Name
Erythrobacter litoralis 222 amino acid protein (EL222)
PR Info
Components
Photoreceptor
Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor
Flavin mononucleotide (FMN)
OS00254
OS Info
OS Name
Enhenced blue light inducible promoter vision 2
[1], [2]
Photoreceptor (PR) Name
Erythrobacter litoralis 222 amino acid protein (EL222)
PR Info
Components
Photoreceptor
Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor
Flavin mononucleotide (FMN)
OS00258
OS Info
OS Name
Light controlled sfGFP expression system
[3]
Photoreceptor (PR) Name
Erythrobacter litoralis 222 amino acid protein (EL222)
PR Info
Components
Photoreceptor
Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor
Flavin mononucleotide (FMN)
OS00259
OS Info
OS Name
Light-regulated stochastic transcriptional regulation system
[4]
Photoreceptor (PR) Name
Erythrobacter litoralis 222 amino acid protein (EL222)
PR Info
Components
Photoreceptor
Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor
Flavin mononucleotide (FMN)
References
1 Cell-Free Optogenetic Gene Expression System
2 Blue light-mediated transcriptional activation and repression of gene expression in bacteria
3 Optogenetics in Sinorhizobium meliloti Enables Spatial Control of Exopolysaccharide Production and Biofilm Structure
4 An Optogenetic Platform for Real-Time, Single-Cell Interrogation of Stochastic Transcriptional Regulation