Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00259
OS Name
Light-regulated stochastic transcriptional regulation system (LRSTR)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein Erythrobacter litoralis 222 amino acid protein
Detail Info
OS Application Information (1)
Mechanism of Action EL222 bind to EL222 binding site and DNA binding [ 1]
Controlled Signal Pathway Transcription regulation
Effect Activation
Application Single-cell interrogation of stochastic transcriptional regualtion
Note For experiments require long-term measurements VP-EL222 may need to be exchanged for another light-sensitive transcription factor; Promoter: EL-bs: EL222 binding site; CYC: truncated CYC1 promoter; PP7 SL-GLT1: PP7 bacteriophage coat protein
Light Information Activation Wavelength 450 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Within 1 min
Light Intensity 11.3 milliwatts per square centimeter
Expression Information Expression Method Transformation
Cell Lines Yeast
References
1 An Optogenetic Platform for Real-Time, Single-Cell Interrogation of Stochastic Transcriptional Regulation