Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00258
OS Name
Light controlled sfGFP expression system (LCsfGFPE)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein Erythrobacter litoralis 222 amino acid protein
Detail Info
OS Application Information (1)
Mechanism of Action EL222 bind to EL222 promoter and DNA binding [ 1]
Controlled Signal Pathway GFP biosynthesis
Effect Activation
Application Using EL222 to optogenetic control GFP biosynthesis in the nitrogen fixing soil bacterium Sinorhizobium meliloti
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 3 s
Deactivation Time 57 s
Light Intensity 6 watts per square meter
Expression Information Expression Method Transformation
Cell Lines Sinorhizobium meliloti cells
References
1 Optogenetics in Sinorhizobium meliloti Enables Spatial Control of Exopolysaccharide Production and Biofilm Structure