Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00254
OS Name	Enhenced blue light inducible promoter vision 2 (PEBLindv2)		[1], [2]
Photoreceptor (PR) PR Info	PR Name Erythrobacter litoralis 222 amino acid protein (EL222) Source of Species Erythrobacter litoralis Class LOV domain UniProt ID Q2NB98 GenBank 333944373 PDB ID 3P7N 3D Structure Components Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222) Cofactor: Flavin mononucleotide (FMN)
Cofactor	Flavin mononucleotide (FMN)
Controlled Protein	Functional protein	Erythrobacter litoralis 222 amino acid protein	Detail Info

OS Application Information (1)
Mechanism of Action	EL222 homodimerization and DNA binding		[ 1] , [ 2]
Controlled Signal Pathway	Gene expression activation
Effect	Activation
Application	A high-throughput screening technology for rapid prototyping of complex biological networks in both space and time
Note	Designed from one component-based blue light-inducible Escherichia coli promoter; Promotor: J23106: weaker constitutive promotor
Light Information	Activation Wavelength	460 nm
	Process	Reversible
	Deactivation Wavelength	Dark
	Activation Time	Seconds
	Deactivation Time	Seconds
	Light Intensity	12 watts per square meter

References

1	Cell-Free Optogenetic Gene Expression System
2	Blue light-mediated transcriptional activation and repression of gene expression in bacteria