Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00699
|
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| OS Name |
Photoactivatable targeted gene editing system
(paCas9-Survivin)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Positive Magnet (pMag) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Functional protein | Negative Magnet | ||||
| Recruited protein | Positive Magnet | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | nMag-pMag heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Survivin gene inhibition | |||||
| Effect | Sensor | |||||
| Application | Survivin gene inhibition | |||||
| Note | Survivin plays the role of anti-apoptosis and is exceedingly apparent in its expression in all tumour cells | |||||
| Light Information | Activation Wavelength | Blue | ||||
| Activation Time | 48 h | |||||
| Light Intensity | 1.2 watts per square meter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | UMUC-3; 5637; T24; SW780; RT4 cells | |||||