Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00280
|
|||||
| OS Name |
Optogenetic regulate Galpha activity system 2
(Opto-Galpha 2)
|
[1] | ||||
|
Photoreceptor (PR) |
||||||
| Complementary Protein | Positive Magnet (pMag) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | Guanine nucleotide-binding protein G(q) subunit alpha | ||||
| Functional protein | Negative Magnet | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | nMag-pMag heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Ca2+ release | |||||
| Effect | Sensor | |||||
| Application | Blue light induced Ca2+ release using Magnet system | |||||
| Note | Won't detect Ca2+ siganal in C71S | |||||
| Light Information | Activation Wavelength | 473 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 50 ms | |||||
| Light Intensity | 1 milliwatts | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK 293 cells | |||||