Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00201
|
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| OS Name |
Optogenetic controlled CAR-T cell signal system
(OptoCAR)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Engineered protein Zdk2 affibody (Zdk2) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Fusion protein | A bacterial SsrA peptide | ||||
| Costmulatory domain of Tumor necrosis factor receptor superfamily member 9 | ||||||
| Recruited protein | Immunoreceptor tyrosine-based activation motifs | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | J-alpha helix unfold and ssrA-SspB heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Light-triggerable production of IL-2 | |||||
| Effect | Activation | |||||
| Application | Optogenetic control CAR T cell for light inducible tumor cell killing | |||||
| Light Information | Activation Wavelength | 470 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 5 s | |||||
| Deactivation Time | Seconds | |||||
| Light Intensity | 1-4 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Jurkat T cells | |||||