Details of the Controlled Protein

General Information of This Controlled Protein (CP)
CP ID CP00473
CP Name Tetracycline repressor protein class D (TetR)
CP Type Recruited protein
Common Name Tetracycline repressor protein class D
Organism Escherichia coli
UniProt ID P0ACT4
UniProt Entry TETR4_ECOLX
Function TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
Sequence
MARLNRESVIDAALELLNETGIDGLTTRKLAQKLGIEQPTLYWHVKNKRALLDALAVEIL
ARHHDYSLPAAGESWQSFLRNNAMSFRRALLRYRDGAKVHLGTRPDEKQYDTVETQLRFM
TENGFSLRDGLYAISAVSHFTLGAVLEQQEHTAALTDRPAAPDENLPPLLREALQIMDSD
DGEQAFLHGLESLIRGFEVQLTALLQIVGGDKLIIPFC
Optogenetic System (OS) Controlling This CP
OS00047
OS Info
OS Name
Light activated rtTA system
[1]
Photoreceptor (PR) Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
PR Info
Components
Photoreceptor
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00653
OS Info
OS Name
Light-inducible cell reprogramming system
[2]
Photoreceptor (PR) Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
PR Info
Components
Photoreceptor
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00605
OS Info
OS Name
Optogenetic control gene expression by green light
[3]
Photoreceptor (PR) Name
Cobalamin-binding domain from Thermus thermophilus (TtCBD)
PR Info
Components
Photoreceptor
Cobalamin-binding domain from Thermus thermophilus (TtCBD)
Cofactor
5-deoxyadenosylcobalamin (AdoCbl)
OS00075
OS Info
OS Name
Photoactivatable tetracycline controlled gene expression system
[4]
Photoreceptor (PR) Name
Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
PR Info
Components
Photoreceptor
Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
References
1 Optogenetic gene editing in regional skin
2 An Optogenetic-Controlled Cell Reprogramming System for Driving Cell Fate and Light-Responsive Chimeric Mice
3 Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes
4 Light Control of the Tet Gene Expression System in Mammalian Cells