Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00011
PR Name Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
Source of Species Arabidopsis thalino
Class Cryptochromes
UniProt ID Q96524
GenBank NP_849588
PDB ID 6K8I
Mutation Site L348F
Sequence
MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSL
AHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYDPVSLVRDHTVKEKLV
ERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPWRLMPITAA
AEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNEFIEKQLIDYAKNSKKVV
GNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRGIGLREYSRYI
CFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMREFWATGWMHNRIRV
IVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYISGSIPDGHELDRLDNPALQG
AKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKPIVDIDTAREL
LAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKEPGLCPSVSSNDQQV
Components Photoreceptor Residue 1-531 of cryptochromes 2 (L348F) (CRY2 (1-531) (L348F))
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Detail Info
Cofactor Flavin adenine dinucleotide (FAD)
Detail Info
3D Structure
Click to Save PDB File
Complementary Protein Detail of This PR
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
UniProt ID A0A061DZ54
Sequence
MNRALPEMLQCSDMTVLERQRARLKWQQEQLQQQQLQQQEQQQSYFSELSGVFSSQPSHV
EGFQGGLMSGDSVLGDMVMTRQLKPDPGLETAWPELVKVDMPGMGFGPCGYGNGPSFDMN
YAISRTSSCPPAVAAAVAGEVVEVKGKESVVSEKIGSAVGRESFKKRKVDKLQNLKVVAE
DDSKRIKACAEEGESKITGPNTNKSSSNNNNNKKESSTDTSKENSKVSEVQKPDYIHVRA
RRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEF
LSMKLAAVNPRLDFNVENLFAKEVFPSCTTNFPTVGMSSEMANPPYLQVSPVQHVVSCCG
LEMGMNTPDMAPRRTISAPVSIPDASFLDSSCFPQIQPSATWDVELQNLYNVAFDQGRST
SFPSQPFTGSIEASNLKMEM
Cofactor Detail of This PR
Cofactor Name Flavin adenine dinucleotide (FAD)
PubChem CID 643975
Structure
Formula C27H33N9O15P2
Molecular Weight 785.5
Optogenetic System (OS) Consisting of This PR
OS00075
OS Info
OS Name: Photoactivatable tetracycline controlled gene expression system (PA-Tet-ON/OFF)
[1]
Recruited protein: Tetracycline repressor protein class D
Detail Info
Fusion protein: Transcription factor p65
Detail Info
OS00074
OS Info
OS Name: Second generation of light-activated BCL2-associated X apoptosis regulator (OptoBax 2.0)
[2]
Fusion protein: BCL2-associated X apoptosis regulator
Detail Info
Recruited protein: Mitochondrial import receptor subunit TOM20
Detail Info
References
1 Light Control of the Tet Gene Expression System in Mammalian Cells
2 Imaging of morphological and biochemical hallmarks of apoptosis with optimized optogenetic tools