Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00543
OS Name
Optogenetic regulate ligand -T-cell receptor interaction system (Opto-ligand-TCR)
 [1], [2], [3]
Photoreceptor (PR)
PR Info
PR Name
Phytochrome B tetramer (PhyBt)
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Phytochrome B tetramer (PhyBt)
Complementary Protein: Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor Phycocyanobilin (PCB)
Controlled Protein Functional protein Phytochrome B
Detail Info
Recruited protein T-cell receptor
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIFS heterodimerization [ 1] , [ 2] , [ 3]
Controlled Signal Pathway Calcium influx
Effect Activation
Application Optical control the kinetic proofreading to regulate the activity of the T cell receptor
Note Light intensity determining the cycling rate
Light Information Activation Wavelength 660 nm
Process Reversible
Deactivation Wavelength 740 nm
Activation Time Seconds
Deactivation Time 2 min
Light Intensity 70 micromoles per square meter per second
Expression Information Expression Method Transfection
Cell Lines Jurkat T cells
References
1 Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor
2 Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System
3 Cross-TCR Antagonism Revealed by Optogenetically Tuning the Half-Life of the TCR Ligand Binding