Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00563
OS Name
Optogenetic controlled iTrkB signaling system 4 (Opto-iTrkB 4)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Aureochromes 1 from Vaucheria frigida (AuLOV)
Source of Species
Vaucheria frigida
Class
LOV domain
UniProt ID
A8QW55
GenBank
BAF91488.1
PDB ID
3UE6
3D Structure
Components
Photoreceptor: Aureochromes 1 from Vaucheria frigida (AuLOV)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Intracellular Tropomyosin receptor kinase B
Detail Info
OS Application Information (1)
Mechanism of Action AuLOV homodimerization [ 1]
Controlled Signal Pathway TrkB signal activation
Effect Sensor
Application Investigate brain-drived neurotrophic factor TrkB signaling with tight spatial and temporal control
Note TrkB palys a critical role in neuronal proliferation, differentiation; survival and death
Light Information Activation Wavelength 470 nm
Activation Time Seconds
Light Intensity 9.7 watts per square centimeter
Expression Information Expression Method Transfection
Cell Lines PC12 cells and NIH 3T3 cells
References
1 Optical Activation of TrkB Signaling