Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00229
OS Name
Versatile method to control protein function by LOVTRAP system 1-1 (LOVTRAP 1-1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 Trapping of Protein (LOVTRAP)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
1047463129
PDB ID
5EFW
3D Structure
Components
Photoreceptor: LOV2 Trapping of Protein (LOVTRAP)
Complementary Protein: Zdark (ZDK)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Zdark (ZDK)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein LOV2 domain of Avena sativa Phototropin 1
Detail Info
Recruited protein Zdark
Detail Info
OS Application Information (1)
Mechanism of Action AsLOV2-Zdk dissociation [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application Versatile method to control protein function with light
Note TOM20 is a mitochondria target sequence
Light Information Activation Wavelength 473 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time 18.5 s
Light Intensity 150 microwatts
Expression Information Expression Method Transfection
Cell Lines HeLa cells; Cos-7 cells; and HEK 293 cells
References
1 LOVTRAP: A Versatile Method to Control Protein Function with Light