Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00200
|
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| OS Name |
LOV2 domain of circular permutants for optogenetic engneering 1
(CPLOV2-1)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Engineered protein Zdk2 affibody (Zdk2) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Recruited protein | Engineered protein Zdk2 affibody | ||||
| Functional protein | LOV2 domain of circular permutants | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | J-alpha helix unfold | [ 1] | ||||
| Controlled Signal Pathway | cpLOV2CZdk2 dissociation | |||||
| Effect | Sensor | |||||
| Application | A modular photoswitch for optogenetic engineering | |||||
| Light Information | Activation Wavelength | 470 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 9.8 ± 0.8 s | |||||
| Deactivation Time | 57.4 ± 3.1s | |||||
| Light Intensity | 4 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HeLa cells; HEK293T cells and NIH3T3 cells | |||||