Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00153
OS Name
Control G-protein signal by LOV2 system (LOV2GIVe)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Girdin
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway GABA activation
Effect Activation
Application Activate the heterotrimeric G-proteins with blue light
Light Information Activation Wavelength 460 ± 40 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 1.5 s
Deactivation Time 1 min
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein