Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00019
OS Name
Optogenetic controlled iTrkB signaling system1 (Opto-iTrkB1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Intracellular Tropomyosin receptor kinase B
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homodimerization [ 1]
Controlled Signal Pathway TrkB signal activation
Effect Sensor
Application Investigate brain-drived neurotrophic factor TrkB signaling with tight spatial and temporal control
Note TrkB palys a critical role in neuronal proliferation, differentiation; survival and death
Light Information Activation Wavelength 470 nm
Activation Time 5 s
Light Intensity 9.7 watts per square centimeter
Expression Information Expression Method Transfection
Cell Lines PC12 cells and NIH 3T3 cells
References
1 Optical Activation of TrkB Signaling