Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00017
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| OS Name |
Light-activated reversible inhibition by assembled trap of intracellular membranes by Rab5
(IM-LARIAT-Rab5)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Functional protein | Cryptochrome-2 | ||||
| Recruited protein | Ras-related protein Rab-5A | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Rab5-positive membrane aggregation | |||||
| Effect | Sensor | |||||
| Application | Dissect spatiotemporal functions of intracellular membranes using Rab5 | |||||
| Note | Can be used to control diverse intracellular membrane by fusing with different protein | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Within seconds | |||||
| Deactivation Time | 5.5 min | |||||
| Light Intensity | 5.5 microwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | COS-7 cells and rat hippocampal neurons | |||||