Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00007
OS Name
Optogenetic control of organelle transport system (Opto-cot)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Protein bicaudal D homolog 2
Detail Info
Fusion protein Transmembrane domain of Miro1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1] , [ 2]
Controlled Signal Pathway Raf1 activation
Effect Activation
Application Transport and distribue organnelles by light
Light Information Activation Wavelength Blue
Process Reversible
Deactivation Wavelength Dark
Activation Time Subseconds
Deactivation Time Minutes
Light Intensity 9.7 watts per square centimeter
Expression Information Expression Method Transfection
Cell Lines COS-7 cells
References
1 Optogenetic control of molecular motors and organelle distributions in cells
2 Light moves mountains in the cell