Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00069
OS Name
Light-Induced Co-clustering (LINC)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6K8I
Mutation Site
E490G
3D Structure
Components
Photoreceptor: Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Clathrin light chain
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homo-oligomerization [ 1]
Controlled Signal Pathway Protein clust
Effect Activation
Application Interrogate protein protein interactions and perturb protein function
Note Longer half-life than wild type with enhanced clustering capability
Light Information Activation Wavelength 461 nm or 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 6 ms
Deactivation Time Minutes
Light Intensity 1.1 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines COS-7 cells and HEK293 cells
References
1 An optimized optogenetic clustering tool for probing protein interaction and function