Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00008
PR Name Photolyase homology region of Cryptochromes 2 (L348F) (CRY2PHR (L348F))
Source of Species Arabidopsis thalino
Class Cryptochromes
UniProt ID Q96524
GenBank NP_849588
PDB ID 6X24
Mutation Site L348F
Sequence
MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSL
AHLSQSLKALGSDLTLIQTHNTISAILDCIRVTGPTKVVFNHLYDPVSLVRDHTVKEKLV
ERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPEEEGQFYPG
RWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNEFIEKQLI
DYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRG
IGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMREFWA
TGWMHNRIRVIVSSFGVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYISGSIPDGHEL
DRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKP
IVDIDTARELLAKAISRTREAQIMIGAA
Components Photoreceptor Photolyase homology region of Cryptochromes 2 (L348F) (CRY2PHR (L348F))
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Detail Info
Cofactor Flavin adenine dinucleotide (FAD)
Detail Info
3D Structure
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Complementary Protein Detail of This PR
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
UniProt ID A0A061DZ54
Sequence
MNRALPEMLQCSDMTVLERQRARLKWQQEQLQQQQLQQQEQQQSYFSELSGVFSSQPSHV
EGFQGGLMSGDSVLGDMVMTRQLKPDPGLETAWPELVKVDMPGMGFGPCGYGNGPSFDMN
YAISRTSSCPPAVAAAVAGEVVEVKGKESVVSEKIGSAVGRESFKKRKVDKLQNLKVVAE
DDSKRIKACAEEGESKITGPNTNKSSSNNNNNKKESSTDTSKENSKVSEVQKPDYIHVRA
RRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEF
LSMKLAAVNPRLDFNVENLFAKEVFPSCTTNFPTVGMSSEMANPPYLQVSPVQHVVSCCG
LEMGMNTPDMAPRRTISAPVSIPDASFLDSSCFPQIQPSATWDVELQNLYNVAFDQGRST
SFPSQPFTGSIEASNLKMEM
Cofactor Detail of This PR
Cofactor Name Flavin adenine dinucleotide (FAD)
PubChem CID 643975
Structure
Formula C27H33N9O15P2
Molecular Weight 785.5
Optogenetic System (OS) Consisting of This PR
OS00068
OS Info
OS Name: Second-generation photoactivatable Cre recombinase (PA-Cre2.0)
[1]
Fusion protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
Recruited protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
References
1 Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase