Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00151
OS Name
Optogenetic control Recombinase Cre by VVD system (Opto-Cre-VVD)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Vivid PAS domain (VVD)
Source of Species
Neurospora crassa
Class
LOV domain
UniProt ID
Q9C3Y6
GenBank
149243087
PDB ID
2PDR
3D Structure
Components
Photoreceptor: Vivid PAS domain (VVD)
Cofactor: Flavin adenine dinucleotide (FAD)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
OS Application Information (1)
Mechanism of Action VVD homodimerization [ 1]
Controlled Signal Pathway Cre activation
Effect Activation
Application Light-inducible recombinases activity control in bacterial
Note nCre and cCre means N and C terminal of the Cre recombinases respectively
Light Information Activation Wavelength 465 nm
Activation Time 1 h
Light Intensity 120 microwatts per square centimeter
Expression Information Expression Method Transformation
Cell Lines E.coli
References
1 Light-Inducible Recombinases for Bacterial Optogenetics