Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00039
OS Name
Optogenetic regulate STE5 activity system (OptoSTE5)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein A single-pass transmembrane protein
Detail Info
Recruited protein MAP kinase scaffold
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1(N) heterodimerization [ 1]
Controlled Signal Pathway GPCR activation
Effect Activation
Application Activate MAP kinase pathway
Note CRY2PHR can activate MAP in significantly reduced background activity
Light Information Activation Wavelength 461 nm
Process Reversible
Deactivation Wavelength Dark
Light Intensity 5.8 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Yeast
References
1 Benchmarking of optical dimerizer systems