Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00009
OS Name
Optogenetic cofilin (OptoCofilin)
 [1], [2], [3]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein A versatile marker
Detail Info
Fusion protein Cofilin; a actin-binding protein
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1] , [ 2] , [ 3]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Optogenetic control subcellular cofilin activity
Light Information Activation Wavelength 488 nm
Process Reversible
Activation Time Seconds
Expression Information Expression Method Transfection
Cell Lines MTLn3 cells
References
1 Optogenetic perturbation of the biochemical pathways that control cell behavior
2 Optogenetic engineering: light-directed cell motility
3 Lifeact: a versatile marker to visualize F-actin