General Information of This Controlled Protein (CP)
CP ID CP00538
CP Name Phytochrome B tetramer (PhyBt)
CP Type Functional protein
Common Name Phytochrome B
Organism Arabidopsis thaliana (Mouse-ear cress)
UniProt ID P14713
UniProt Entry PHYB_ARATH
KEGG ath:AT2G18790
Function Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenetic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion. Involved in the flowering time regulation. Involved in light-regulated circadian phase control that triggers stomatal aperture, stomatal conductance, and CO2 assimilation. Implicated in red light perception, and, to a lower extent, in blue light signaling. Controls thermomorphogenesis in the daytime and regulates temperature responses by associating with the promoters of key target genes in a temperature-dependent manner and subsequently repressing their expression in a PIF4-dependent manner (temperature-responsive transcriptional regulator); this process requires PTAC12/HMR/PAP5 (transcriptional activator). Thermal timer that integrates temperature information over the course of the night. Detabilizes UNE10/PIF8 in red light.
Sequence
MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQ
YTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCM
IAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFV
AREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVR
AISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIG
LHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQ
YMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEF
LMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAA
FLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMA
VAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWE
TAEMDAIHSLQLILRDSFKES
Optogenetic System (OS) Controlling This CP
OS00690
OS Info
OS Name
Optogenetic T Cell Receptor controling system 1
[1]
Photoreceptor (PR) Name
Phytochrome B tetramer (PhyBt)
PR Info
Components
Photoreceptor
Phytochrome B tetramer (PhyBt)
Complementary Protein
Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor
Phycocyanobilin (PCB)
OS00691
OS Info
OS Name
Optogenetic T Cell Receptor controling system 2
[1]
Photoreceptor (PR) Name
Phytochrome B tetramer (PhyBt)
PR Info
Components
Photoreceptor
Phytochrome B tetramer (PhyBt)
Complementary Protein
Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor
Phycocyanobilin (PCB)
OS00692
OS Info
OS Name
Optogenetic T Cell Receptor controling system 3
[1]
Photoreceptor (PR) Name
Phytochrome B tetramer (PhyBt)
PR Info
Components
Photoreceptor
Phytochrome B tetramer (PhyBt)
Complementary Protein
Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor
Phycocyanobilin (PCB)
OS00693
OS Info
OS Name
Optogenetic T Cell Receptor controling system 4
[1]
Photoreceptor (PR) Name
Phytochrome B tetramer (PhyBt)
PR Info
Components
Photoreceptor
Phytochrome B tetramer (PhyBt)
Complementary Protein
Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor
Phycocyanobilin (PCB)
OS00694
OS Info
OS Name
Optogenetic T Cell Receptor controling system 5
[1]
Photoreceptor (PR) Name
Phytochrome B tetramer (PhyBt)
PR Info
Components
Photoreceptor
Phytochrome B tetramer (PhyBt)
Complementary Protein
Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor
Phycocyanobilin (PCB)
References
1 Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts