Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00124
PR Name LOV2 Trapping of Protein (C450A; L514K; G528A; L531E; N538E) (LOVTRAP (C450A; L514K; G528A; L531E; N538E))
Source of Species Avena sativa
Class LOV domain
UniProt ID O49003
GenBank 1047463129
PDB ID 5EFW
Mutation Site C450A; L514K; G528A; L531E; N538E
Sequence
LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNARFLQGPETDRATV
RKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQKDGTEHVRDA
AEREAVMEIKKTAEEIDEAAKEL
Components Photoreceptor LOV2 Trapping of Protein (C450A; L514K; G528A; L531E; N538E) (LOVTRAP (C450A; L514K; G528A; L531E; N538E))
Complementary Protein Zdark (ZDK)
Detail Info
Cofactor Flavin mononucleotide (FMN)
Detail Info
3D Structure
Click to Save PDB File
Complementary Protein Detail of This PR
Complementary Protein Zdark (ZDK)
Sequence
GSVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANLLAEAKKLNDAQAPK
Cofactor Detail of This PR
Cofactor Name Flavin mononucleotide (FMN)
PubChem CID 643976
Structure
Formula C17H21N4O9P
Molecular Weight 456.3
Optogenetic System (OS) Consisting of This PR
OS00244
OS Info
OS Name: Optogenetic control alphaTAT activity by LOVTRAP system 2 (OptoalphaTAT-LOVTRAP 2)
[1] , [2]
Fusion protein: Alpha-tubulin acetyl transferase
Detail Info
OS00243
OS Info
OS Name: Optogenetic control coffilin activity by LOVTRAP system 2 (OptoCOF-LOVTRAP 2)
[1] , [2]
Fusion protein: Cofilin; a actin-binding protein
Detail Info
References
1 Optogenetic control of cofilin and TAT in living cells using Z-lock
2 Engineering Optogenetic Protein Analogs