Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00243
OS Name
Optogenetic control coffilin activity by LOVTRAP system 2 (OptoCOF-LOVTRAP 2)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
LOV2 Trapping of Protein (C450A; L514K; G528A; L531E; N538E) (LOVTRAP (C450A; L514K; G528A; L531E; N538E))
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
1047463129
PDB ID
5EFW
Mutation Site
C450A; L514K; G528A; L531E; N538E
3D Structure
Components
Photoreceptor: LOV2 Trapping of Protein (C450A; L514K; G528A; L531E; N538E) (LOVTRAP (C450A; L514K; G528A; L531E; N538E))
Complementary Protein: Zdark (ZDK)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Zdark (ZDK)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Cofilin; a actin-binding protein
Detail Info
OS Application Information (1)
Mechanism of Action AsLOV2-Zdk dissociation [ 1] , [ 2]
Controlled Signal Pathway Cofilin activation
Effect Sensor
Application Use Z-lock to opto-control cofilin activity in living cells
Note Light state comparason
Light Information Activation Wavelength Lit state mutant
Process Ireversible
Expression Information Expression Method Transfection
Cell Lines MTLn3 cells
References
1 Optogenetic control of cofilin and TAT in living cells using Z-lock
2 Engineering Optogenetic Protein Analogs