Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00725
OS Name
opto-TLR4-LOV LECs (opto-TLR4-LOV LECs)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Aureochromes 1 from Vaucheria frigida (AuLOV)
Source of Species
Vaucheria frigida
Class
LOV domain
UniProt ID
A8QW55
GenBank
BAF91488.1
PDB ID
3UE6
3D Structure
Components
Photoreceptor: Aureochromes 1 from Vaucheria frigida (AuLOV)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Toll-like receptor 4
Detail Info
OS Application Information (1)
Mechanism of Action AuLOV homodimerization [ 1]
Controlled Signal Pathway TLR4-related pro-inflammatory signaling pathways
Effect Activation
Application Release diverse pro-inflammatory mediators, beneficial in controlling bacterial infections
Light Information Activation Wavelength 470nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Expression Information Expression Method Transfection
Cell Lines Human lymphatic endothelial cells (LECs)
References
1 Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial Cells