Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00723
OS Name
Bue light-inducible nuclear export system - s 3 (iLEXYs 3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (V416L) (AsLOV2 (V416L))
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
Mutation Site
V416L
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (V416L) (AsLOV2 (V416L))
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein NTF2-related export protein 1
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Nxf1 (TAP); Ran-GTP; CRM1
Effect Sensor
Application Export factor
Note The mutations slow down the dark reversion rate
Light Information Activation Wavelength Blue
Process Reversible
Deactivation Wavelength Dark
Activation Time 30 min
Deactivation Time t1/2 = 636.6 s
Expression Information Expression Method Transfection
Cell Lines HEK 293E cells
References
1 Analysis of Slow-Cycling Variants of the Light-Inducible Nuclear Protein Export System LEXY in Mammalian Cells