Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00672
OS Name
Optogenetic induction of caspase-8 mediated apoptosis V2 (Opto-Casp8-V2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Caspase-8
Detail Info
Recruited protein Caspase-8
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR-CIBN heterodimerization [ 1]
Controlled Signal Pathway Caspase-8 activation
Effect Activation
Application Cell apoptosis
Light Information Activation Wavelength Blue
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Deactivation Time Minutes
Expression Information Expression Method Transfection
Cell Lines HEK293T; HeLa cells
References
1 Optogenetic induction of caspase-8 mediated apoptosis by employing Arabidopsis cryptochrome 2