Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00658
OS Name
Light-gated I-BAR (inverse BAR) domain containing tools 5 (CRYARs 5)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein CAAX prenyl protease?
Detail Info
Fusion protein I-BAR (inverse BAR) domain (MTSS1(1-250))
Detail Info
WH2 ((MTSS1(601-759))
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR-CIBN heterodimerization [ 1]
Controlled Signal Pathway Remodeling of membrane architectures
Effect Activation
Application Deformation and protrusion formation of the cellular membrane
Note Ezrin is an important mediator of switch function
Light Information Activation Wavelength 480 nm or 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Deactivation Time 30 min
Expression Information Expression Method Transfection
Cell Lines HEK293T
References
1 CRY-BARs: Versatile light-gated molecular tools for the remodeling of membrane architectures