Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00628
OS Name
Optogenetic dopamine transporter multimerization system (OptoDTM)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Sodium-dependent dopamine transporter
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 oligomerization [ 1]
Controlled Signal Pathway Dopamine transportation
Effect Activation
Application Test the functional importance of DAT multimerization in both the absence and pressence of ligands for DAT
Note DAT multimers may function to help maitain DA homeostasis
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 30 s
Deactivation Time Minutes
Light Intensity 13 microwatts
Expression Information Expression Method Transfection
Cell Lines HEK293 cells
References
1 Optogenetically-induced multimerization of the dopamine transporter increases uptake and trafficking to the plasma membrane