Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00622
|
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| OS Name |
Optogenetic control Exoc70 for membrane traffiking system 1
(Opto-Exoc70-MT 1)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | Exocyst complex component 70 | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Exocytosis | |||||
| Effect | Activation | |||||
| Application | Optotigenetic control Exo70 to study the role of the exocyst in tethering during exocytosis | |||||
| Note | CIBN was tethered on membrane | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Minutes | |||||
| Deactivation Time | Minutes | |||||
| Light Intensity | 1.5 watts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Hela cells | |||||