Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00621
OS Name
Optogenetic recruit talin for integrin activation system (OptoRTIA)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Talin-1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Integrin activation
Effect Activation
Application Activate intergrin by recruitment of full length talin to the plasma membrane
Note Integrin activation needs both membrane recruitment of talin and membranea ssociated Ras-related protein 1 (Rap1)CGTP
Light Information Activation Wavelength 450 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 30 min
Light Intensity 50 miliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines CHO cells
References
1 Optogenetics-based localization of talin to the plasma membrane promotes activation of 3 integrins