Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00599
OS Name
Optogenetic control nucleo/cytoplasmatic partitioning system (Opto-NCP)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue1-908 of Phytochrome B (PhyB (1-908))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
Components
Photoreceptor: Residue1-908 of Phytochrome B (PhyB (1-908))
Complementary Protein: Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Fusion protein Nuclear export sequence
Detail Info
Functional protein Phytochrome B
Detail Info
Recruited protein Tyrosine-protein kinase ZAP-70
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF3 heterodimerization [ 1]
Controlled Signal Pathway Nuclear import and export
Effect Sensor
Application Dynamic control of nucleo/cytoplasmatic partition
Light Information Activation Wavelength 660 nm
Process Reversible
Deactivation Wavelength 740 nm
Activation Time 30 min
Deactivation Time 50 min
Light Intensity 20 micromoles per square meter
Expression Information Expression Method Transfection
Cell Lines NIH 3T3 cells
References
1 Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish