Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00535
OS Name	Optogenetic control transcription activation system 2 (Opto-CTA 2)		[1]
Photoreceptor (PR) PR Info	PR Name Residue1-621 of Phytochrome B (PhyB (1-621)) Source of Species Arabidopsis Class Phytochromes UniProt ID P14713 GenBank NP_179469 PDB ID 4OUR 3D Structure Components Photoreceptor: Residue1-621 of Phytochrome B (PhyB (1-621)) Complementary Protein: Phytochrome interacting factor 3 (PIF3) Cofactor: Phycocyanobilin (PCB)
Complementary Protein	Phytochrome interacting factor 3 (PIF3)
Cofactor	Phycocyanobilin (PCB)
Controlled Protein	Recruited protein	Activation domain	Detail Info
Controlled Protein	Fusion protein	DNA Binding Domain	Detail Info

OS Application Information (1)
Mechanism of Action	PhyB-PIF3 heterodimerization		[ 1]
Controlled Signal Pathway	Gene expression activation
Effect	Activation
Application	Genetically encoded PhyB optogentics using undogenously produced PCB
Note	Fd+FNR system is the rate-limiting factor for the production of the chromophores PCB in the mitochondria of mammalian cells
Light Information	Activation Wavelength	660 nm
	Process	Reversible
	Deactivation Wavelength	735 nm
	Activation Time	Minutes to Hours
	Deactivation Time	Hours
	Light Intensity	0.1-1 micromoles per square meter per second
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	HEK293 cells; HUH cells; Hela cells and 3T3 cells

References

Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded PhyB Optogenetics