Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00527
|
|||||
| OS Name |
Light-activated nuclear translocation of adeno-associated virus nanoparticles system 1
(LANT-AAV 1)
|
[1] | ||||
|
Photoreceptor (PR) |
||||||
| Complementary Protein | Phytochrome interacting factor 6 (PIF6) | |||||
| Cofactor | Phycocyanobilin (PCB) | |||||
| Controlled Protein | Recruited protein | Adeno-associated virus nanooparticles VNP-1,2 | ||||
| Functional protein | Phytochrome B | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | PhyB-PIF heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Gene dilivery activation | |||||
| Effect | Sensor | |||||
| Application | Light-regulated translocation of AAV nanoparticles and spatially programmable gene delivery | |||||
| Note | This protein-virus hybrid system addresses the common gene delivery barrier of nuclear entry | |||||
| Light Information | Activation Wavelength | 650 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | 750 nm | |||||
| Activation Time | Seconds | |||||
| Deactivation Time | Seconds | |||||
| Light Intensity | < 10 micromoles per square meter per second | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Hela cells; human mesenchymal stem cells; human umbilical vein endothelial cells and 3T3 fibroblasts | |||||