Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00514
|
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| OS Name |
Optogenetic controlled SOS signaling system
(OptoSOS)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Phytochrome interacting factor 3 (PIF3) | |||||
| Cofactor | Phycocyanobilin (PCB) | |||||
| Controlled Protein | Recruited protein | Nucleotide exchange factor SOS | ||||
| Functional protein | Phytochrome B | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | PhyB-PIF3 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | SOS activity regulation | |||||
| Effect | Sensor | |||||
| Application | Precise control SOS mediated cell signals by light | |||||
| Note | Cotransfected with PIF-SOScat and PHYB-mCherry-CaaX ratio 1:10; cross-activation of PHYB by blue light can be avoided by concurrent illumination with 740 nm light | |||||
| Light Information | Activation Wavelength | 660 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | 740 nm | |||||
| Activation Time | 15 min | |||||
| Deactivation Time | Minutes | |||||
| Light Intensity | 0.9 or 1.8 watts per square meter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK293 cells | |||||