Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00305
|
|||||
| OS Name |
Optogenetic control opsin activity by luciferase 1
(Opto-LF1)
|
[1] | ||||
|
Photoreceptor (PR) |
||||||
| Cofactor | All-trans-Retinal (Trans-Retinal) | |||||
| Controlled Protein | Functional protein | Volvox channelrhodopsin-1 | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | Ion channel open | [ 1] | ||||
| Controlled Signal Pathway | Neuron activation | |||||
| Effect | Activation | |||||
| Application | GLucM23 is an efficienrt light source for activating optogenetic elements in bioluminescence driven optogenetics | |||||
| Note | GLucM23=GLuc mutation at K25E, M35L, V88D, M102I, and G159D | |||||
| Current Intensity | 954 ± 340 pA | |||||
| Channel Type | Cation channel | |||||
| Light Information | Activation Wavelength | 470 nm | ||||
| Activation Time | 1 s | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Mouse hippocampal neurons | |||||