Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00302
|
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| OS Name |
Design Channelrhodopsin 2 for ultrafast optogenetic control system 1
(ChR2-UOC 1)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Cofactor | All-trans-Retinal (Trans-Retinal) | |||||
| Controlled Protein | Functional protein | Channelrhodopsin 2 | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | Ion channel open | [ 1] | ||||
| Controlled Signal Pathway | Neuron activation | |||||
| Effect | Activation | |||||
| Application | Design channelrhodopsin 2 by mutation for ultrafast modify neuron activity | |||||
| Note | Experimental control | |||||
| Current Intensity | 10 nA | |||||
| Channel Type | Cation channel | |||||
| Light Information | Activation Wavelength | 500 ± 25 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 55.5 ms | |||||
| Deactivation Time | 9.8 ms | |||||
| Light Intensity | 50 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Rat hippocampal neurons | |||||