Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00296
|
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| OS Name |
MCh tag Channelrhodopsin2 for neuron signal regulation system 1
(MCh-ChR2-NSR 1)
|
[1], [2] | ||||
|
Photoreceptor (PR) |
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| Cofactor | All-trans-Retinal (Trans-Retinal) | |||||
| Controlled Protein | Functional protein | Channelrhodopsin 2 | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | Ion channel open | [ 1] | ||||
| Controlled Signal Pathway | Hcrt release | |||||
| Effect | Activation | |||||
| Application | Optogenetic control hypocretin neurons to prove the impact of hcrt neuron activity on sleep state transition | |||||
| Note | Hcrt receptor 1 antagonist will block the photostimulation for both SWS and REM sleep-to-awake transitions | |||||
| Current Intensity | 182 ± 86 pA | |||||
| Channel Type | Cation channel | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Activation Time | 1 s for current detect and 10 s for sleep test | |||||
| Light Intensity | 1 milliwatts per square miliimeter | |||||
| Expression Information | Expression Method | Transduction | ||||
| Cell Lines | Hcrt (Hypocretin) neuron cells | |||||
| OS Application Information (2) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | Ion channel open | [ 2] | ||||
| Controlled Signal Pathway | GABAA-mediated Cl- conductance | |||||
| Effect | Inhibition | |||||
| Application | Selectively control fast-spiking interneurons by optogenetic manipulation | |||||
| Current Intensity | 53-mV | |||||
| Channel Type | Cation channel | |||||
| Light Information | Activation Wavelength | 473 nm | ||||
| Process | Reversible | |||||
| Activation Time | 3 s | |||||
| Light Intensity | 31 or 46 or 68 milliwatts per square miliimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Adult CW2 mice inhibitory interneurons in Barrel cortex | |||||