Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00293
OS Name
EYFP tag Channelrhodopsin2 for neuron signal regulation system 1 (EYFP-ChR2-NSR 1)
 [1], [2], [3], [4]
Photoreceptor (PR)
PR Info
PR Name
Channelrhodopsin 2 (ChR2)
Source of Species
Chlamydomonas reinhardtii
Class
Opsin
UniProt ID
Q8RUT8
GenBank
1304111922
PDB ID
6EID
3D Structure
Components
Photoreceptor: Channelrhodopsin 2 (ChR2)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Channelrhodopsin 2
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 2]
Controlled Signal Pathway Enhanced 5-HT release
Effect Activation
Application Optogenetic stimulate 5-HT in median raphe nucleus (MRN) and dorsal hippocampus (dHC) to regulate anxiety-like behaviour
Channel Type Cation channel
Light Information Activation Wavelength 473 nm
Activation Time 10 ms
Light Intensity 10 milliwatts
Expression Information Expression Method Transgene
Cell Lines Mouse median raphe serotonin neurons
OS Application Information (2)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Insulin secretion activation
Effect Activation
Application Control insulin secretion by pancereatic Beta-cells to treat typediabetes
Note Longer time than 60 s will damage cell
Channel Type Cation channel
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 5 s
Light Intensity 50 microjoules
Expression Information Expression Method Transfection
Cell Lines MIN6 cells
OS Application Information (3)
Mechanism of Action Ion channel open [ 3]
Controlled Signal Pathway Compulsive behavior regulation
Effect Sensor
Application Evaluate rats compulsive behavior
Note Increase connectivity in the OFCCVMS pathway may not be sufficient for compulsivity
Channel Type Cation channel
Light Information Activation Wavelength 465 nm
Activation Time 5 min
Light Intensity 2C5 milliwatts per square miliimeter
Expression Information Expression Method Transfection
Cell Lines Rat orbitofrontal cortex ventromedial striatum
OS Application Information (4)
Mechanism of Action Ion channel open [ 4]
Controlled Signal Pathway Traumatic brain injury recovery
Effect Sensor
Application Study Traumatic brain injury (TBI) effect on cortex
Note The cerebral cortex in the brain area most affected by traumatic brain injury
Channel Type Cation channel
Light Information Activation Wavelength 470 nm
Activation Time 5 s
Expression Information Expression Method Transgene
Cell Lines Adult male sprague dawley rats cortex
References
1 Optogenetic control of insulin secretion by pancreatic -cells in vitro and in vivo
2 Median raphe serotonin neurons promote anxiety-like behavior via inputs to the dorsal hippocampus
3 Lost in translation: no effect of repeated optogenetic cortico-striatal stimulation on compulsivity in rats
4 Reorganization of Thalamic Inputs to Lesioned Cortex Following Experimental Traumatic Brain Injury