Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00235
OS Name
Versatile method to control protein function by LOVTRAP system 3-1 (LOVTRAP 3-1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 Trapping of Protein (V416T) (LOVTRAP (V416T) )
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
1047463129
PDB ID
5EFW
Mutation Site
V416T
3D Structure
Components
Photoreceptor: LOV2 Trapping of Protein (V416T) (LOVTRAP (V416T) )
Complementary Protein: Zdark (ZDK)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Zdark (ZDK)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein LOV2 domain of Avena sativa Phototropin 1
Detail Info
Recruited protein Zdark
Detail Info
OS Application Information (1)
Mechanism of Action AsLOV2-Zdk dissociation [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application Versatile method to control protein function with light
Note TOM20 is a mitochondria target sequence
Light Information Activation Wavelength 473 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time 5.0 s
Light Intensity 150 microwatts
Expression Information Expression Method Transfection
Cell Lines HeLa cells; Cos-7 cells; and HEK 293 cells
References
1 LOVTRAP: A Versatile Method to Control Protein Function with Light