Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00172
OS Name
Circularly permuted LOV inhibitor of protein synthesis 2 (CLIPS 2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein CRISPR-associated endonuclease Cas9
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Translation inhibition
Effect Inhibition
Application Optogentic inhibitors of eukaryotic translation initiation
Note AsLOV2=A'alpha--Jalpha
Light Information Activation Wavelength 450 nm
Process Reversible
Deactivation Wavelength Thermal
Activation Time 30 s
Deactivation Time Minutes
Light Intensity 50 microwatts per square centimeter
Expression Information Expression Method Transformation
Cell Lines Yeast
References
1 A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation Initiation