Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00167
|
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| OS Name |
Optogenetic control protein-protein interaction system 4
(Opto-PPI 4)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Recruited protein | Binder of AsLOV2-dark state | ||||
| Functional protein | LOV2 domain of Avena sativa Phototropin 1 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | BoLL-AsLOV2 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Sensor | |||||
| Application | Phage display to discover selective binder for photoswitchable proteins for protein localization | |||||
| Note | BoLL bind to light state AsLOV2 only | |||||
| Light Information | Activation Wavelength | 450 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | <3 s | |||||
| Deactivation Time | 3 min | |||||
| Expression Information | Expression Method | Transfection and Transformation | ||||
| Cell Lines | E. coli K12 | |||||